FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396074
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32110.23000213455733723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53950.041.232851
GGTATCA20600.039.3890341
TATTCTA1006.3034113E-638.51947470
GTGGTAT7300.037.6788521
TATCAAC59700.037.2615172
AACGCAG67100.033.0497176
TGGTATC8250.032.9233252
ATCAACG69000.032.13623
AGAGTAC67700.031.89573711
CAACGCA69650.031.8397125
CAGAGTA68400.031.5704510
TCAACGC70800.031.368864
ACGCAGA72000.030.7560547
GCAGAGT71850.030.6288349
CGCAGAG74300.029.665148
TGTATAA2600.027.770042
GTGTATA2450.026.6635551
GTATAAG2900.024.8946023
AGTACGG78900.024.4473613
GAGTACG80750.023.97242512
Produced by FastQC (version 0.11.5)