FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306960
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16020.12257452408642958No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT15970.12219195690763299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3100.045.2388731
TATACAC2950.045.211523
TTATACA3200.041.679372
GTATCAA53650.040.9949951
ATAACCG350.001407320739.083955
GGTATCA21750.039.0015641
CTCCTAT3400.036.40795532
ACTCCTA3500.036.31244331
TATCAAC61900.035.690152
ATACACA3850.035.5308654
GTACTAA501.9578947E-434.2050061
CTCGTAT3550.032.17547239
TACACAT4150.032.138315
AACGCAG69550.031.8136636
GTGGTAT7050.031.536531
CAACGCA70850.031.2299275
AATCTAT450.004232795431.22421853
TCCTATC3750.031.20000333
GAATCTA450.00425400631.19234552
ATCAACG72350.030.6769873
Produced by FastQC (version 0.11.5)