FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118271
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58930.5269742307544414No Hit
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA51970.4647352922502685No Hit
AGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCC48370.43254273785155833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43240.3866683478333964No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT39100.3496469102748797No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTTCCAAGACTCTGC37150.33220927664224503No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36650.3277380885313131No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35290.31557645686957814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33620.30064268857906534No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32940.2945618727481979No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31830.2846358351419289No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30330.271222270809133No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA29920.2675558965581688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29540.26415779359386055No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC28960.2589712153851794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28560.2553942648964339No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28150.25172789064546963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27270.2438585995702294No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA24800.2217709303022255No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24360.21783628476460537No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC24250.21685262338020034No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAG23350.20880448478052277No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT23240.20782082339611774No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23180.20728428082280592No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22990.20558522934065177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22620.2022765501385621No Hit
GTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCT22520.2013823125163757No Hit
TTCCAAGACTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC21570.19288705510560497No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA21280.19029376600126446No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG20300.1815302373038378No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTTCCA19450.17392921751525345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19310.1726772848441925No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18960.16954745316654013No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18930.16927918187988422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18490.1653445363422641No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18300.16364548486010994No Hit
CTCCTGCAGAGTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAAT18260.16328778981123537No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC18240.1631089422867981No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA18150.16230412842683034No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC17920.16024738189580165No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG17790.15908487298695934No Hit
GTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCC17780.15899544922474068No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17570.15711755021814927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17510.15658100764483743No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGT17250.1542559898271528No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16980.15184154824724955No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16960.15166270072281227No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16410.1467443938007871No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15630.13976934034773325No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT15620.1396799165855146No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15600.13950106906107734No Hit
CTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGGGAGACTGGAGA15450.13815971262779772No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCC15410.13780201757892316No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15370.13744432253004862No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGG15040.13449333837683353No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAC14950.13368852451686578No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14930.1335096769924285No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA14730.1317212017480557No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA14710.1315423542236184No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA14400.12877021759484059No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGA14210.12707116611268646No Hit
CTCCAGGGGTGACGGGCAGGGAGACTGGAGACTGAGTCATTACAATATCC14120.1262663522527187No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTC14100.12608750472828142No Hit
CTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGC13980.12501441958165774No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13870.12403075819725272No Hit
ACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGC13680.12233170671509858No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13660.12215285919066131No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13660.12215285919066131No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13450.12027496018406987No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13400.11982784137297667No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13190.11794994236638526No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13110.11723455226863615No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13030.11651916217088702No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12950.11580377207313791No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12920.11553550078648199No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12580.11249509287104824No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCT12420.11106431267555003No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12240.10945468495561451No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA12050.10775563347346037No Hit
GTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCCCTGA11980.1071296671379299No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11790.10543061565577574No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACT11750.1050729206069012No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11470.10256905526477929No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11240.10051230873375058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC200.00606027154.35379854
GCCGTCT5350.044.0681446
TATACAC6500.043.948673
TCGTATG6050.043.88953440
TATGGCG404.7266803E-543.54038643
GGTATCA10700.042.5302051
CGTATGC5900.042.21228441
TTATACA7000.041.2821662
CTTATAC7000.040.8094831
CCGTCTT5800.040.80175447
GTCGTAT1350.040.73828539
TGCCGTC6050.039.9924145
ATGCCGT6300.039.390344
ACGAGAC7550.038.7529222
ATCTCCG7650.038.20530310
TCTCCGA7700.037.9572211
CTTGAAA6850.037.50319757
GTATCAA24850.037.2939571
CACGAGA7900.037.03104421
GTGGTAT3000.036.5387231
Produced by FastQC (version 0.11.5)