FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192389
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC252.589379E-455.1040571
CTTAGGA200.007413574551.6535573
TACTAGT200.00741480451.651392
TATACAC352.2981449E-549.1938633
GGTATCA11700.035.9118351
GTATCAA24650.035.907061
TATCAAC26500.033.5246732
TATTCCC652.3513865E-531.7868025
CCCCTAG450.00424187231.21034459
AGCATAA450.0044693830.87889544
AAGCATA450.004484202730.85800643
CCAGTAA450.00460135230.69577233
ATAGATT450.004664175730.61079824
CTAGACT450.00466513330.6095183
TTATACA450.0046660930.6082322
TATAGGG450.0046660930.6082322
GTATATG500.007800969327.5520291
TTAGGAA500.007805760527.5485634
CCTAGAC500.00780735827.5474072
CAGAGTA31600.027.23886710
Produced by FastQC (version 0.11.5)