FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871998
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14970.17167470567593046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT150.002345745569.0711532
GCTCTAA200.007079816452.2576249
GTGGTAT3650.046.187341
GTATCAA31400.043.8278431
TATCAAC33150.041.405622
GGTATCA11000.039.7219731
CAATAGT350.001366828339.3153274
TGGTATC4400.039.091942
CAGAGTA37900.035.49419410
AGAGTAC37950.035.4474311
AACGCAG39750.034.621246
TTTAGCA400.002582899834.53557632
CGTTTAG400.002600448234.48792630
TCGTTTA400.002605584234.47405629
GAACTAT501.8914966E-434.4048586
AATAGAG400.002632865434.4009132
GCAGAGT39550.034.3613629
ACGCAGA40950.033.60677
CAACGCA40900.033.5617375
CGCAGAG41300.033.1552858
Produced by FastQC (version 0.11.5)