FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143733
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.038.2104721
GTATCAA44900.037.839731
GTGGTAT7350.035.1658741
TATCAAC51700.032.857382
ATCAACG53950.031.4870553
CAACGCA54350.031.1919215
AACGCAG54750.031.026976
TCAACGC55000.030.8859354
TGGTATC8100.030.2029862
ACGCAGA59250.028.6684847
AGAGTAC57950.028.5380211
CAGAGTA58700.028.17339310
CGCAGAG60300.027.9400778
TATAAGA5700.025.3893152
TCAAACG6500.025.13191846
GCAGAGT68250.024.6855189
AGTACGG54650.024.08348713
GAGTACG55450.023.611212
ATAAGAG6300.022.9712853
GTATAAG7300.022.1882211
Produced by FastQC (version 0.11.5)