FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143733
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25760.1201642182118762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44100.037.005561
GGTATCA19650.036.9698031
GTGGTAT8200.034.4292221
TGGTATC9300.030.3484522
TATCAAC53250.030.2504542
ATCAACG54900.029.2145333
AACGCAG54450.029.0148566
TCAACGC55650.028.6971114
CAACGCA55750.028.6463055
AGAGTAC59200.026.74619911
CAGAGTA59550.026.58899910
ACGCAGA61200.025.8152927
CGCAGAG61500.025.6893638
AGTACGG55200.023.1968913
GCAGAGT68350.023.165159
GTAGTTT8900.022.72328270
GAGTACG56700.022.64392312
TCAAACG7750.022.42905246
TACGGGG26250.020.32394615
AACGTTA1600.004822350619.74972770
Produced by FastQC (version 0.11.5)