FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397007
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAAT300.003111762564.3017470
TAGTTTA350.00572926755.11578470
TTATACA507.165727E-848.095112
TATACAC507.165727E-848.095113
GTGTATA451.9118452E-645.8106351
TCGTATG503.720077E-641.5857740
ACGGATA350.001329813639.53016338
GTATCAA10550.038.7544981
GGTATCA4350.036.3325771
CTCGTAT601.308916E-534.6196239
GTGGTAT1600.034.357981
ATACACA807.343806E-834.3536534
TACACAT905.3914846E-934.353655
TAAGACT1400.031.899829
TATCAAC13150.031.0880972
CTAAGAC1450.030.7998268
CTTATAC802.8456834E-630.0632321
ATCAACG13750.029.9813693
CGATATG604.353662E-429.8420267
CAACGCA13950.029.5515275
Produced by FastQC (version 0.11.5)