FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397007
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7690.19369935542698238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC200.007472687451.54351819
GTATCAA11350.039.035321
TCTAGTG451.0295445E-438.1467255
GGTATCA5100.032.9981651
TATCAAC13700.032.3272632
CCGAATT553.3279145E-431.24243522
CTAGTGA553.3476486E-431.2109576
CAGAGTA13900.030.8741510
GTTCAGA1252.8672388E-530.8599870
AGAGTAC14050.030.5445311
AACGCAG14500.030.5436866
GTGGTAT2250.030.5289151
CAACGCA15000.029.7544465
ATCAACG15050.029.1993523
CGCAGAG15050.029.1993528
GCAGAGT15050.029.1993529
ACGCAGA15700.028.6464927
TCAACGC15550.028.260474
TTAGTAT500.00784245527.51762628
GTGTATG500.00790058227.476021
Produced by FastQC (version 0.11.5)