FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312512
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42420.32319704505558805No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28430.21660754339769844No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27700.21104568948702943No Hit
GTATGGTAGCTCCCCTCCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27510.20959808367466354No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATG26760.20388385020479813No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGGTACC25920.19748390871854887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25480.1941315584162278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24340.18544592354203238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22360.17036034718158766No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGCGGAGGGGAGCTACCATACTG22090.16830322313243612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21800.16609371952408816No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT20980.1598461575970353No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20850.15885569046225864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20780.1583223620050712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18660.1421701287302516No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAA18020.13729398283596647No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT17620.13424639165203822No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17540.13363687341525257No Hit
CTACCATACTGCTGACAGAAATACATTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16760.12769407060659255No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16410.12502742832065536No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATGTTGCAATGTATTTCTGTCAGCAGTATGGTAGCTCC16010.12197983713672714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15050.11466561829529939No Hit
CATCTATGGTGCATTCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15000.11428466939730837No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14630.11146564755217475No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14500.11047518041739808No Hit
CAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGA14380.10956090306221962No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14190.1081132972498537No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14010.10674188121708601No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13960.10636093231909498No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13820.10529427540472011No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13500.10285620245757753No Hit
GTATTTCTGTCAGCAGTATGGTAGCTCCCCTCCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13480.10270382289838112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.040.042591
TATACAC2750.038.7672653
GTATCAA32550.038.2525141
TTATACA2900.037.9479372
TCGTATG2700.037.2128340
CTTATAC2850.036.2058531
ATACACA3300.034.390324
TATCAAC36850.033.7837032
CTAGCGA500.003418585532.6165169
TACACAT3400.032.3673555
TGTTAGA6850.031.74881770
AACGCAG40100.031.0456246
CAACGCA40600.030.8326975
TTAACGG901.9805157E-730.6931435
AGAGTAC40650.029.94191711
ATCAACG41750.029.9010413
CGTATGC3400.029.5774841
TCAACGC42150.029.5356924
GTATGCC3550.029.32595442
GCAGAGT42800.028.9198239
Produced by FastQC (version 0.11.5)