FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421332
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25270.17779097353749862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.040.3890231
GTATCAA54150.039.9583741
TATCAAC59500.036.2974472
GTGGTAT6950.033.6573371
AACGCAG66450.032.292956
CAACGCA68900.031.2444765
AGAGTAC66450.031.10485511
ATCAACG69750.030.8637163
CAGAGTA67100.030.75228710
TCAACGC71100.030.3260654
TGGTATC7400.030.2075182
GCAGAGT70800.029.9709559
CGCAGAG72750.029.451258
ACGCAGA72800.029.3837837
GTCTAGG1155.8025762E-826.9215661
TAGTATC658.822732E-426.452994
GTGTATA1801.2732926E-1124.8442861
AGTACGG75900.024.69468113
GAGTACG76300.024.65536712
AAAACGT851.4666945E-424.29587724
Produced by FastQC (version 0.11.5)