FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14540.12570742849633512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13270.1147274811654998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36300.040.4279861
GGTATCA15100.037.0997921
TATCAAC42600.034.2086452
CAACGCA46600.031.3460355
AACGCAG46700.031.2789136
ATCAACG47150.030.9074943
TCAACGC47900.030.8540764
CGTAATC605.2713027E-428.88899241
AGAGTAC50000.028.6583911
CGCAGAG51200.028.3247288
ACGCAGA52050.027.9954327
GCAGAGT51850.027.9009559
TCGTATG1004.8400034E-727.71409240
TATACTG1800.026.7322275
GTGTATA2350.026.3248231
TATACAC2000.025.7775043
TCGCTAT951.0823573E-525.3241731
CAGAGTA56900.025.18311910
GTGGTAT6250.024.7453351
TGGTATC6550.024.1377142
Produced by FastQC (version 0.11.5)