FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17470.15103911800763237No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14170.12250854620309963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36700.038.9269521
GGTATCA16100.038.6078721
TATCAAC42600.033.6910632
GTGGTAT7100.032.8907431
CAACGCA45750.031.4464025
AACGCAG45900.031.3449926
TCAACGC46750.030.9193084
TGGTATC7450.030.8792382
ATCAACG46600.030.8714773
AGAGTAC48600.028.47192611
CGCAGAG50500.028.2845978
GCAGAGT50600.028.0929859
TGTTAGA4300.028.07261370
ACGCAGA51750.027.8016457
TATACTG1306.6356733E-926.4122095
CGATTGT1751.0523176E-525.41310570
CAGAGTA55350.025.30990810
AGTACGG47400.023.54252613
GAGTACG48700.023.196112
CCTATAC1403.954883E-722.0721043
Produced by FastQC (version 0.11.5)