FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929923
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42070.2179879715408335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41270.2138427284404611No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38540.19969708636044026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32640.16912591849519384No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31840.16498067539482145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31190.16161266537576888No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25250.13083423535550381No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24390.1263780990226035No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23640.12249193361600437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20770.1076208739934184No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19890.10306110658300875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19430.10067759180029462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71450.037.8703541
GGTATCA31050.036.629121
CCTAGAC853.4779077E-935.9464153
CTTATAC3100.035.045441
TATACAC3350.033.4426883
TATCAAC85750.031.7512652
TCGTATG3150.030.7615140
AACGCAG90500.029.9730016
TAATCTC2800.029.41503535
CAACGCA94450.028.971115
TCAACGC96350.028.3998054
AGAGTAC93700.028.292811
ATCAACG97000.028.1044983
CTCGTAT2950.028.1033839
CAGAGTA95350.027.83880210
GCAGAGT97650.027.6704719
CGCAGAG99700.027.2050728
GTGGTAT12850.025.627831
ACGCGTA3800.025.0712127
TACACAT4500.024.8962215
Produced by FastQC (version 0.11.5)