FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929923
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC37060.19202838662475136No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36940.1914066001596955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32650.1691777340339485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32440.16808960772010076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31740.1644625200072749No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31210.16171629645327817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30990.1605763546006758No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA25880.1340986142970471No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24310.12596357471256625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23350.12098928299211938No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23320.1208338363758554No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19740.10228387350168892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74050.038.0932161
GGTATCA31550.036.966511
TATCAAC87200.032.4585652
AACGCAG91450.030.8381086
ATCAACG95600.029.6042563
CAACGCA96200.029.4556085
TCAACGC97150.029.097044
AGAGTAC94600.028.91698311
CAGAGTA95250.028.79082510
GCAGAGT98400.028.5236999
CGCAGAG98900.028.4815798
CTAGAGA1900.026.7585934
TAGACTG1058.9710375E-725.8248335
ACGCAGA113050.025.006617
ATACGTA1951.8189894E-1224.3368311
AGTACGG83100.023.45449613
GAGTACG83950.023.2170212
GTGCTAG2157.2759576E-1222.0769441
TGTGTAA1500.002615503721.94844270
ATTCACT4050.021.760942
Produced by FastQC (version 0.11.5)