FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973936
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17710.18183946378406796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17140.17598692316538253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16380.16818353567380198No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15100.15504098831956103No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15050.154527607563536No Hit
GTCTACAAACTCCTAGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14990.15391155065630596No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14570.14959915230569565No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14200.14580013471111036No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13350.13707266185868477No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12780.13122012123999935No Hit
GTATAGAACCCACTCCAGAAATCGTACGTCGATCTCTCGCACAGTAATACACGGCCGTGTCCTCGGCTCTCAGGC12000.12321138144600877No Hit
GTTCTATACCCTCCAATGAAACTCCTGCCCCCGGCTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCT11690.12002842075865354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11600.11910433539780849No Hit
GTGTATTACTGTGCGAGAGATCGACGTACGATTTCTGGAGTGGGTTCTAT11120.11417588013996813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11030.11325179477912307No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10650.10935010103333279No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10590.10873404412610276No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA10530.10811798721887268No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10170.10442164577549244No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10170.10442164577549244No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10000.10267615120500731No Hit
CCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAGATCAGGAGCTGTGGAGACTGCCCTGG9750.10010924742488213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)