FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973936
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17510.1797859407599678No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17070.17526819010694747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15220.15627310213402112No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14790.15185802763220582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14560.14949647615449063No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13990.14364393553580523No Hit
GTCTACAAACTCCTAGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA13980.14354125938460022No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13550.13912618488278491No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT13490.13851012797555487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13050.13399237732253452No Hit
GTTCTATACCCTCCAATGAAACTCCTGCCCCCGGCTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCT12860.1320415304496394No Hit
GTGTATTACTGTGCGAGAGATCGACGTACGATTTCTGGAGTGGGTTCTAT12200.1252649044701089No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11920.12238997223636872No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11770.12084982996829362No Hit
GTATAGAACCCACTCCAGAAATCGTACGTCGATCTCTCGCACAGTAATACACGGCCGTGTCCTCGGCTCTCAGGC11510.11818025003696342No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11320.11622940316406828No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10680.10965812948694781No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10280.10555108343874751No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA10230.10503770268272249No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10180.10452432192669743No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10050.10318953196103235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.039.534981
GGTATCA7200.032.43661
TATCAAC18950.032.4316372
AACGCAG19150.032.0962226
ATCAACG19150.031.9103583
CAACGCA19450.031.4213925
TCAACGC19350.031.2273034
TGTTAGA5350.031.2261870
TATTATA1001.51922E-830.9006462
TAGATAC605.5767305E-428.611715
AGAGTAC21650.027.91271811
CAGAGTA21750.027.7858110
GTGGTAT3100.027.6972851
CGCAGAG21900.027.5954978
CTATTAT1005.2182077E-727.4757061
ACGCAGA23300.026.3795137
TGTGCGA5100.024.2382910
ACGTACG4850.024.09149724
GTATTAC5000.023.3447573
GACGTAC5350.023.12109423
Produced by FastQC (version 0.11.5)