FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363188
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30010.22014571724516355No Hit
GTGTATTACTGTGCGGGGTCTGTCGCCCGAGACTTGGAGTGGTTATTTGG29430.21589098495585346No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21150.1551510136532892No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20840.15287693260210625No Hit
GTAATACACAGCCGTGTCTGCGGCACTCACAGAGGTCAGCTTCAGGGAAA19620.14392732330390232No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19560.14348717858431853No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18950.13901237393521657No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16850.12360730874978358No Hit
GTCTCTGGTGGCTCCGTCACCAGTAATATTTACTACTGGGGCTGGATCCG15180.11135661405470118No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13720.10064642587816208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT7650.040.7954970
GTATCAA24250.037.627431
GTGGTAT5050.036.6806531
TATCAAC27150.033.089552
ATCAACG27900.031.9519023
TCAACGC28600.031.1710034
CAACGCA28800.030.7186815
AACGCAG29250.030.4850236
GCACGGA7850.028.46003769
AGAGTAC31350.028.33356911
CAGAGTA31800.027.82579610
GTATACG500.00795437127.4425621
CGCAGAG32900.026.9996958
GCAGAGT33100.026.8365579
ACGCAGA33650.026.4998427
GACGTGT7600.025.14001362
TCGGACG8100.024.34384559
AGTACGG30600.023.75864213
GAGTACG31350.023.62780612
GGTATCA15450.022.4247471
Produced by FastQC (version 0.11.5)