FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021709
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATA150.002364880668.93066426
TATACAC1600.055.9705243
CTTATAC1550.055.559311
CTAAGCG200.00702996452.35174653
TTATACA1650.052.1869742
ATACACA1900.048.945884
TAGGACT305.91074E-446.615855
TTTAGAT608.6232804E-443.17151670
GTATCAA33750.042.8670961
GGTATCA12150.041.1093181
GGACTTA650.001278487339.85062870
TATCAAC37600.038.3824042
TACACAT2450.037.958035
GTGGTAT3950.035.754881
GAGACTC2650.035.1035824
CGAGACT2900.034.45351823
AGGGGGG1705.5079E-934.2832670
CAGAGTA41300.033.60777710
AGAGTAC41350.033.48384511
CAACGCA43850.033.0688065
Produced by FastQC (version 0.11.5)