FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322330
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20100.1520044164467266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAA200.007165186652.10096443
TATGCTA200.00754296451.428325
GGTATCA17900.040.5633281
GTATCAA42150.039.652721
TAAACTA1800.038.0489355
CAATTAA1850.037.0261881
TATCAAC47000.035.627032
GTGGTAT5750.035.1426851
ATTAAAC2100.034.244043
CTTATAT400.002693533234.242752
ATGTAAG450.003196223933.06765468
AAACTAT2100.032.615846
TTAAACT2100.032.6133734
GTATAAG959.447831E-932.4466361
CAGAGTA51200.031.4361510
AGAGTAC51150.031.3999311
AGTTACG850.00428976131.13770
GCAGAGT53400.030.9747149
AACGCAG53850.030.971426
ATACAGT450.00479265630.4414526
Produced by FastQC (version 0.11.5)