FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805465
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGAG200.0071539552.1197843
TATTAAC200.00744575251.5954742
TATACAC605.7589205E-945.8626443
TAATCAA551.5039768E-743.7779775
TAAACTA1450.042.6997035
ATTAAAC1450.042.6997033
AGACTAG651.1654265E-842.34526425
TTATACA651.1679731E-842.3347472
ATACACA651.1679731E-842.3347474
CAATTAA1500.041.271251
GGTATCA9600.040.8413431
ATAATCA602.9781768E-740.1298144
TTCTTAT2600.040.0610470
GTATCAA24350.039.8305741
GAGACTA702.2413587E-839.32060624
GTGGTAT3500.039.3059541
TCATAAT801.6880222E-938.6966062
TTAAACT1650.037.5239834
TATCAAC27450.035.46212
TCGCTTA400.00237835635.1210459
Produced by FastQC (version 0.11.5)