FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805465
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12120.15047208755191102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG1250.046.7523841
TCATAAT405.1111507E-542.9656072
GTGGTAT4400.041.408361
ATAAGAG1350.040.735233
TAAACTA1200.040.103725
TAAGCGT350.001267991139.91975451
GGTATCA11900.039.287721
ATAATCA350.001372749739.2804034
GTATCAA27400.039.26981
ATTAAAC1250.038.4947933
TTAAACT1250.038.4947934
AAACTAT1300.037.0234226
TAAGAGA1600.036.5184974
TATCAAC29750.036.3943982
TATAAGA1550.035.4812772
GTCTTAG400.002641830834.3767551
CTACTCT400.002644244834.3703544
CAATTAA1450.033.1913451
GTCATAA751.7156835E-632.084971
CAGAGTA33550.031.76588810
Produced by FastQC (version 0.11.5)