FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656610
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC408.311472E-751.703443
TACACAT506.988557E-848.2565425
CAATTAA652.1464075E-1047.7335241
TTATACA451.8717401E-645.958622
TGTTTAC306.337515E-445.9586142
TTATACT306.337515E-445.9586144
TTAAACT605.649781E-945.9586144
ACTAGAT306.342275E-445.9516116
GGTATCA7150.045.8048931
GTGGTAT1900.045.3608061
GTATCAA18750.043.0238151
AAACTAT651.1461452E-842.4233366
ATTAAAC651.1461452E-842.4233363
ACTATGC955.456968E-1239.902318
TACAGTT350.001284847739.81159251
GTGTTTA350.001352357839.3990971
TAAACTA702.2042514E-839.3930975
TATCAAC20750.038.7049062
GCTATTG700.001817599237.0931470
GAGACTA601.3506735E-534.46633524
Produced by FastQC (version 0.11.5)