FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656610
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10300.15686632856642452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAG200.007413859551.6504553
GTATCAA17850.047.662181
TCATAAT306.369616E-445.911522
TATCAAC19700.043.5227172
GGTATCA5750.043.1300351
GTGGTAT1800.040.1848141
CAATTAA955.456968E-1239.8826751
TTAGCAT350.001336944639.49106234
TAAACTA1009.094947E-1237.8775
AGAGTAC22100.037.5526611
CAGAGTA22200.037.5386210
CAACGCA23700.036.3224035
GCAGAGT23500.036.1947029
AACGCAG23700.036.1798676
ATTAAAC1051.6370905E-1136.073333
TTAAACT1051.6370905E-1136.073334
GTCATAA400.002616237834.444131
AAACTAT1102.910383E-1134.436266
TTAGACA400.002620149634.4336364
TAAGAGA400.002620149634.4336364
Produced by FastQC (version 0.11.5)