FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336838
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44300.18957240510467563No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42490.1818268960022047No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27340.11699570102848379No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26600.11382902879874428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26280.11245965702372179No Hit
GTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT25630.10967812060570738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25480.10903622758616559No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23960.10253171165480876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62150.039.4035571
GGTATCA26450.038.1776051
TATCAAC69950.034.999252
GTGGTAT10300.034.3468631
ATCAACG72600.033.577693
TCAACGC73500.033.1198164
AACGCAG75100.032.5534486
CAACGCA74900.032.547995
TGGTATC11450.030.2881572
AGAGTAC82450.029.31956511
ACGCAGA83400.029.2737987
CAGAGTA83400.029.02676210
CGCAGAG84350.028.9848088
AGTACGG64400.024.9004113
GAGTACG65350.024.74861112
GCAGAGT103000.024.1033069
TTACTCG5650.022.20073148
TACGGGG33100.021.8882715
CATACGA953.166049E-421.68718310
GCGTTGT7650.021.66305562
Produced by FastQC (version 0.11.5)