FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539677
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9340.17306648235889247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT200.007335587451.78801332
TATTACA200.007444082851.596082
CGGTAAT400.00880267249.4473970
TAATCAA405.081554E-543.0047045
AATCAAT405.081554E-543.0047046
GTATCAA16100.039.3368151
GGTATCA6800.038.975261
TCATAAT451.0185695E-438.219322
GTATAAG557.506138E-637.548781
TAAACTA853.0449883E-936.4275135
ATTAAAC853.0504452E-936.420763
TTAAACT853.0504452E-936.420764
GTGGTAT2750.036.297151
TATCAAC17750.035.4632232
CAATTAA851.2313285E-732.3950231
GTCTATG652.3564855E-531.7720411
CAGAGTA19800.031.44990510
AGAGTAC19850.031.37068611
GCAGAGT20750.030.8390359
AACGCAG21000.030.6357296
Produced by FastQC (version 0.11.5)