FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427104
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTA401.0773128E-682.3998870
TTAGTCT150.00223380969.9303350
CTAACCC150.002416797468.5555954
TAGAATA200.00652459253.3497564
ACTAGCA200.007549821451.4166952
TTTAGTT305.727789E-446.91537557
ATTTTTA305.8228645E-446.7589554
CTTATAC1100.046.7473641
TTATACA1100.046.742452
TTTTTAG350.001237375240.1208155
TAGCAAG350.001379244839.24476228
TATACAC1400.039.1746253
TACACAT1400.039.1746255
CGCTAAT1350.038.23919732
TGCTATA451.0405312E-438.0864452
CTAATCT1009.094947E-1237.9435834
GTATCAA45050.037.7437861
TCACTAA450.002190322435.71714869
GGTATCA19650.035.240931
TATCAAC48900.034.9086762
Produced by FastQC (version 0.11.5)