FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427104
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23320.16340785254613538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTA306.5369287E-445.6742325
GTATCAA45550.038.816431
TATCAAC48950.035.9002272
GGTATCA19000.034.8063131
GTGGTAT8100.034.2652781
TACGGTA501.9410302E-434.2556722
TGGTATC8400.033.0322572
AACGCAG58150.030.3382156
CAGAGTA56050.030.25361810
AGAGTAC56150.030.19973811
GTATAAG802.9132134E-629.982121
GCAGAGT58450.029.7732969
CAACGCA60400.029.1513545
ACGCAGA61350.028.6451137
CGCAGAG60950.028.5520828
TCAACGC62700.028.2459094
CTACACA854.680962E-628.2105544
ATCAACG63100.028.0668533
ACTGTGT756.468681E-527.40558
TAAGAGA951.1105534E-525.2410264
Produced by FastQC (version 0.11.5)