FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451864
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2050.041.911181
GGTATCA5850.039.3606871
GTATCAA12650.036.4047471
TGGTATC2550.033.6858442
CTTATAC851.2471901E-732.345571
ATAGCAT552.776279E-432.2237965
TCCTTAG552.8878427E-432.00818360
CTACCGT553.302518E-431.28443328
TATCAAC14800.030.8771722
AGTAAGA900.00662540128.48049570
TTTATGC500.006999205328.16720460
GTTAATA500.0078769127.4937361
TAGATTG500.00788116627.4906925
TATACAC1005.1830284E-727.4906923
AACGCAG16500.027.2824296
TATAGCA657.5032614E-427.19177664
TGGCTAA906.6119574E-626.9778842
GCTAAAG658.2836964E-426.73518244
TAGCATC807.80295E-526.65461766
TCAACGC16850.026.5117934
Produced by FastQC (version 0.11.5)