FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775532
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21840.2816131378202318No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21460.2767132755321508No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20460.2638189010898325No Hit
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAACTCAGCTTTAGGGAGA17790.22939092132884267No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17090.22036485921921983No Hit
GAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT16000.20630999107709289No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15930.2054073848661306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15260.19676815398977734No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14950.19277089791265867No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTT14930.1925130104238123No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14410.18580793571380677No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13760.17742659232629987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13470.17368722373802759No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13020.16788475523898433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12750.1644032741395584No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.15976129934032382No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11820.15241150590820238No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11780.15189573093050962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11590.14944579978646916No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGGTACTTTCTACTGGACCTGGATCCG11340.14622220617588957No Hit
TCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACT11330.14609326243146642No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11140.14364333128742593No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10920.1408065689101159No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA10790.13913030023261452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10740.1384855815104986No Hit
GTGTATTACTGTGCGAGAGATGTGTGGGCGGTAGGACCAGCTGCGCGAAT10720.13822769402165222No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10580.13642248159972767No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC10130.1306200131006844No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10070.12984635063414532No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9960.12842796944549031No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9360.12069134478009934No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9060.11682303244740384No Hit
CTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACTCTCA8910.11488887628105611No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8770.11308366385913153No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8740.11269683262586198No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8610.1110205639483606No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8550.11024690148182151No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8540.11011795773739834No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8500.1096021827597056No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8410.10844168905989696No Hit
GATCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCA8390.10818380157105059No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8350.10766802659335785No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8250.10637858914912601No Hit
CAGTAGAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGTTAATGCTCTGA8210.10586281417143328No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8200.10573387042701012No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC8200.10573387042701012No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7920.10212344558316098No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7860.10134978311662189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.037.8973431
GTGGTAT2450.036.3505171
GTGTATA400.00269045934.2489513
TATCAAC19100.032.812332
TGTTAGA4550.032.2405970
GTATAAG1101.1423253E-931.139431
TAATTAG450.00478683930.447441
GTATTAA550.0048643730.34675469
ATCAACG20800.030.2971483
TCAACGC21050.029.7746244
AACGCAG21300.029.4251566
AGAGTAC21250.029.16640511
CAACGCA21900.028.7753755
CGCTGAT8550.028.34679870
CGCAGAG22150.028.294158
TGTATAA500.00801141827.40092714
TATAAGA1005.347629E-727.3973942
GCAGAGT22700.027.3051159
CACAGTA4700.027.1405570
ACGCAGA23400.026.9290667
Produced by FastQC (version 0.11.5)