FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775532
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21180.2731028506883017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20340.26227157615675434No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17390.22423317155191533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16270.2097914721765188No Hit
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAACTCAGCTTTAGGGAGA15860.2045047786551683No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTT15680.20218379125555103No Hit
GAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT15070.19431822284573685No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14760.19032096676861818No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14450.1863237106914995No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13710.17678187360418396No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13450.1734293362491812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13440.17330039250475804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13350.1721398988049494No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13050.16827158647225388No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12200.15731136819628333No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11710.15099312471954734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11690.15073523723070098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11350.14635114992031276No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11280.14544854370935048No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11160.1439012187762723No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGGTACTTTCTACTGGACCTGGATCCG11040.14235389384319408No Hit
GTGTATTACTGTGCGAGAGATGTGTGGGCGGTAGGACCAGCTGCGCGAAT11020.14209600635434774No Hit
TCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACT10610.13680931283299722No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10360.13358571922241763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10020.1292016319120294No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9890.12752536323452804No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9860.1271385320012585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9750.1257201508126035No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA9700.12507543209048755No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC9540.12301233217971662No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9440.12172289473548481No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9300.11991768231356023No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9150.1179835261472125No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9060.11682303244740384No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8900.11475993253663291No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8660.11166528267047653No Hit
CTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACTCTCA8590.11076267645951424No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8510.10973112650412879No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8500.1096021827597056No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8430.1086995765487433No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8310.10715225161566512No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8230.10612070166027966No Hit
GATCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCA8200.10573387042701012No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC8130.10483126421604783No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8030.10354182677181599No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7870.10147872686104506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)