FastQCFastQC Report
Wed 8 May 2024
EGAF00005760284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900668
Total Bases67.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23530.26125053848921026No Hit
GAGTTCACCCTCACCATCAATATGCTGGAAACTGAAGATGCTGCAACGTA19370.2150625979828305No Hit
GTATTACTGTCATCAGAGTCGGAGTTTACCAGGGACTTTTGGCCAGGGGA16930.1879715944165886No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16840.18697233608832556No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT16110.17886724075908103No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15190.16865260007017013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14690.16310116491315335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14210.15777178716241724No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13780.1529975529273828No Hit
CAGTAATACGTTGCAGCATCTTCAGTTTCCAGCATATTGATGGTGAGGGT12360.1372314770814551No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12300.1365653048626131No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11860.1316800419244383No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC11290.12535140584543916No Hit
GTGTAAGCTATTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT11090.12313083178263244No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG10920.12124334382924674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10900.12102128642296607No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10750.11935585587586103No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10380.11524779385966862No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10050.11158384665603753No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA9880.10969635870265182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9380.10414492354563502No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9280.10303463651423167No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9120.1012581772639863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)