FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047497
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40050.3823399971551231No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27410.26167139380828774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26510.253079483759858No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25950.24773340639639066No Hit
GTATAATAACTGGCCTCCGAGGTACACTTTTGGCCAGGGGACCAAGCTGG24750.23627752633181764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22770.21737532422527223No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21550.2057285128262897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21180.20219628313971305No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20920.1997141757923889No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20360.19436809842892153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20060.19150412841277825No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17470.1667785206067416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16740.15980952690079303No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCGGTGGCCCTGGC15600.1489264408394487No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15400.1470171274953532No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15310.14615793649051023No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15090.14405769181200517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14760.14090732479424764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14520.13861614878133302No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14010.1337473997538895No Hit
GTTATTATACTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT13390.12782852838719347No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13380.1277330627199887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13230.1263010777119171No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCTCGGAGGCCAGTTATTATA12530.11961848100758284No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12150.11599078565380139No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCAGTATAATAACTGG12070.11522706031616319No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12010.11465426631293454No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11850.11312681563765815No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11600.11074017395753877No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10700.10214826390910904No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10620.10138453857147084No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10500.10023895056501354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22000.036.2696271
CGCTAAT958.880306E-932.65202332
ATCAACG25200.031.7898163
CAACGCA26400.030.3448245
TCAACGC26350.030.2719214
TATCAAC26600.030.1181052
CTTATAC1151.7607817E-929.907491
TAACGGC703.8426042E-529.58052636
AACGCAG27250.029.3982896
TCGTATG1103.569221E-828.30240240
AGAGTAC28800.027.69670911
CGCAGAG28950.027.55328
GTCGAAC550.00825158627.2361868
TCGCTAA1155.7267243E-826.95664431
ACGCAGA30250.026.3690977
GCAGAGT30650.025.9127889
TTAACGG809.56281E-525.87428335
GGTATCA17050.025.2152581
ATGCCGT1251.2005148E-724.97327844
CGTATGC1251.2247801E-724.92167341
Produced by FastQC (version 0.11.5)