FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601040
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAG98200.6133513216409334No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAAT95170.594426123020037No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC80000.4996752111127767No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT70170.4382776195472942No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG69750.4356543246889522No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCTTGATTTATGAGGGCA67400.42097636536251437No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG64590.40342527357217806No Hit
CCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCC59970.37456903013041526No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC55620.34739919052615803No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53670.33521960725528405No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAGTCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC53470.33397041922750215No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51500.32166591715385No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAGTCTTGTCTCCTGGTACCAACACCA46680.2915604856843052No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46080.28781292160095934No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG44750.2795058212162095No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC44030.2750087443161945No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC43900.2741967720981362No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG43900.2741967720981362No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG41860.2614550542147604No Hit
GTGTTGGTACCAGGAGACAAGACTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA41380.25845700294808377No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41340.25820716534252736No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTGGCACTAAGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT39340.24571528506470794No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC38370.23965672312996553No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA38030.23753310348273624No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT37860.23647129365912156No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC37710.23553440263828512No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG37300.23297356718133214No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36060.2252286014090841No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34230.2137985309548793No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTTAGTGCCACTAC33680.210363263878479No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33210.2074276720131914No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC32850.20517913356318396No Hit
GTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACG32440.20261829810623097No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32210.20118173187428173No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG32150.20080697546594717No Hit
GCACTAAGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC31520.19687203317843402No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30610.1911882276520262No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30060.18775296057562585No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29380.18350572128116724No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT29350.18331834307699996No Hit
ACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC29300.18300604607005447No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC29090.18169439864088344No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA28660.17900864438115224No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28410.17744715934642483No Hit
GTTTGGTCCCTCCGCCGAACACCTTAGTGCCACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAG28170.17594813371308649No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG28050.17519862089641733No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27600.17238794783390796No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27150.16957727477139858No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC27130.1694523559686204No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTGGGAGCC26740.1670164393144456No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT26490.16545495427971818No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT26030.16258182181581973No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT25720.1606455803727577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25010.15621096287413183No Hit
TTGCCAGACTGGGAGCCAGAGAAGCGATTAGAAACCCATGAGGGCCGCTG24650.1539624244241243No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCTTAGTGCCA24580.15352520861440064No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA24400.1524009393893969No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24350.1520886423824514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24280.15165142657272773No Hit
GTTATAGTCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA23620.14752910608104733No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC23530.14696697146854545No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23180.14478089241992706No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23140.14453105481437067No Hit
TATGCAGGTAGTGGCACTAAGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22980.1435317043921451No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC22940.14328186678658872No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC22850.14271973217408684No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22520.14065857192824663No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT22510.14059611252685755No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC21790.13609903562684253No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21780.13603657622545345No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG21740.13578673861989707No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21570.13472492879628242No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21440.13391295657822413No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20460.12779193524209265No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC19700.12304502073652125No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTGGCA19680.12292010193374306No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19600.12242042672263029No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19410.12123369809623745No Hit
CCCATGAGGGCCGCTGACTGCCCTCATAAATCAAGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA19070.11911007844900814No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC18600.1161744865837206No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC18530.11573727077399691No Hit
GTCAGCGGCCCTCATGGGTTTCTAATCGCTTCTCTGGCTCCCAGTCTGGC18310.11436316394343676No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18300.11430070454204767No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18250.1139884075351022No Hit
CTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC18160.11342627292260032No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17520.1094288712336981No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17500.10930395243091989No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTGGGAGCCAGAGAAGCGATTAGAAACCCATGAGGGCCG17460.10905411482536351No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC17330.10824214260730526No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA17300.10805476440313795No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG17220.10755508919202518No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17180.1073052515864688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17090.1067431169739669No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17090.1067431169739669No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC17070.10661819817118874No Hit
GCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA17010.10624344176285415No Hit
ACACCACCCAGGCAAAGCCCCCAAACTCTTGATTTATGAGGGCAGTCAGCGGCCCTCATGGGTTTCTAATCGCTT16900.10555638834757407No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTA16880.10543146954479587No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT16720.10443211912257032No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG16630.10386998451006847No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16600.10368260630590116No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAG16450.10274571528506471No Hit
GAGTTATAGTCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCA16450.10274571528506471No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16430.10262079648228652No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTC16360.10218358067256283No Hit
ACCCAGGCAAAGCCCCCAAACTCTTGATTTATGAGGGCAGTCAGCGGCCC16310.10187128366561736No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC16260.10155898665867187No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16260.10155898665867187No Hit
TCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTGGGAGCCAGAGAAGCGATTAGAAACC16230.10137160845450456No Hit
GCACTGGAACCAGCAGTGATGTTGGGAGTTATAGTCTTGTCTCCTGGTAC16150.1008719332433918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.043.1149141
GTGGTAT5000.042.0229841
TTCGTAT650.001106125441.0440770
TGGTATC5450.039.154582
GTATCAA33700.038.2268681
TATCAAC37950.033.737942
CGATTGT1201.693257E-533.3483170
ATCAACG39000.032.8029443
TCAACGC39150.032.671144
CAACGCA40350.031.7847215
AACGCAG41000.031.2808156
CGCAGAG43000.029.8258938
TCGAGTA703.7702117E-529.6645141
CAGAGTA44000.029.22800310
AGAGTAC44000.029.14894311
GCAGAGT44750.028.8122889
ACGCAGA46000.027.8807287
GCGTAGA4000.026.67864870
TTCTAAT41250.026.67864670
TTGACGT809.703303E-525.82006528
Produced by FastQC (version 0.11.5)