FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613279
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTC100460.6227069217413727No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCATAAGTACCA93470.5793790162767878No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC78010.4835493426741438No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC65560.40637732221147116No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT59230.367140463614787No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG58990.36565281020827767No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG58120.3602600666096813No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54490.3377593088362273No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG53030.3287094172799621No Hit
CCTCATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCG50900.3155064932971916No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC50620.31377089765626404No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50000.30992779302278156No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC49090.3042871071897669No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC49070.30416313607255785No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC46030.2853195262567727No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG45480.2819103205335221No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG45440.28166237829910384No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT43620.2703810066330746No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43400.2690173243437744No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT42610.26412046521401444No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC42490.26337663851075976No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG40950.2538308624856581No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA38970.24155772188195596No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCATAAGTACCAC38630.23945021288940102No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC38390.2379625594828917No Hit
GTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG36840.22835479789918545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35840.22215624203872983No Hit
GGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG34690.21502790279920586No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34590.21440804721316029No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG34360.2129823793652555No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33970.21056494257967778No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGAGGTGTTCGG33480.20752765020805453No Hit
CCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCT33380.20690779462200895No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTT32980.2044283722778267No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32610.20213490660945813No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT31880.1976099608313255No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31530.19544046628016606No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31340.19426274066667948No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA31040.19240317390854278No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30610.18973779488854686No Hit
CATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT30220.18732035810296918No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC29630.18366321014530035No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAG29340.18186562894576822No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28240.17504721749926702No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC27750.17200992512764376No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT27510.1705222717211344No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT27270.16903461831462507No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26860.16649321041183826No Hit
CTTATGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC26760.16587335482579268No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26350.16333194692300587No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26290.16296003357137856No Hit
CACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT25660.1590549433792915No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC25380.15731934773836392No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC25100.15558375209743636No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25050.15527382430441355No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24580.15236050304999943No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24290.15056292185046727No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24200.15000505182302626No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC24130.14957115291279438No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAG24000.14876534065093514No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23930.14833144174070326No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATGAGGTGTTCGGCG23930.14833144174070326No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT23480.14554209160349824No Hit
GTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGC23200.14380649596257064No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCATAAGTAC23050.1428767125835023No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC22860.1416989869700157No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22750.14101714582536562No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22650.14039729023932004No Hit
GCTTGGTCCCTCCGCCGAACACCTCATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT22650.14039729023932004No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCA22530.13965346353606536No Hit
AGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCC22320.1383517668053697No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA21830.13531447443374642No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21380.1325251242965414No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA21230.13159534091747305No Hit
GTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGG20580.1275662796081769No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20570.12750429404957234No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGAC20280.1257067128500402No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20260.1255827417328311No Hit
GGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGC19720.12223552156818504No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCC19550.12118176707190757No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT19460.12062389704446658No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19420.12037595481004836No Hit
CTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGA19360.12000404145842102No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCT19360.12000404145842102No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19250.11932220031377089No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATT19190.11895028696214356No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC19150.11870234472772534No Hit
GGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT19140.11864035916912077No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT18980.11764859023144787No Hit
GGGATCCCTGAGGGCCTCTCA18890.11709072020400686No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18850.11684277796958865No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC18500.11467328341842917No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT18420.11417739894959272No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC18240.1130616588947107No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAG17880.11083017878494668No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17840.11058223655052847No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC17690.10965245317146012No Hit
GTACTTATGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT17570.10890862646820544No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATA17420.10797884308913709No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT17400.10785487197192797No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17310.10729700194448696No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17120.1061192763310004No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCA16930.10494155071751385No Hit
GCAGTGGTACTTATGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA16910.10481757960030473No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16890.10469360848309561No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16890.10469360848309561No Hit
GGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGC16730.10370183954542271No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16680.10339191175239994No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16680.10339191175239994No Hit
CTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAG16560.10264808504914524No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC16480.1021522005803088No Hit
GCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAG16470.10209021502170423No Hit
GGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTC16270.10085050384961312No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16150.10010667714635843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTTA9450.050.4222831
TCTTTAT9650.048.630562
CTTTATA9800.048.5506443
TTATATA9800.048.541615
TATATAT10350.045.962116
TTTATAT10550.044.7663964
GTGGTAT3850.044.519351
ATATATC11000.042.9350437
GGTATCA14150.042.8801541
TGGTATC4150.041.270332
ATATCAC12150.039.4358069
GTATCAA33250.038.558441
TATCACC13400.035.50168210
TATCAAC37600.033.8899652
TCAACGC39850.032.2053834
ATCAACG40300.032.0215643
CAACGCA40400.031.851655
AACGCAG41100.031.4757046
ATCACCA15250.031.4242211
ATACACG450.00415327831.34590158
Produced by FastQC (version 0.11.5)