FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988352
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59290.5998874894774331No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41090.4157425694489412No Hit
GAGTTACAGTACCCCTATGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT40070.4054223596451466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35940.36363562779252734No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35920.363433270737551No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35240.3565531308683546No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32690.3307526063588681No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA32440.32822314317166357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31740.3211406462474908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31570.31942061128019167No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31510.3188135401152626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28350.286841125428997No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC27970.28299634138444607No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG27460.27783623648254874No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27320.27641973709771417No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGAAT27040.2735867383280451No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25940.262457100304345No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAA25270.2556781389626368No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24980.2527439616654795No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23820.24100725247685034No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23320.23594832610244124No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23080.23352004144272487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22660.22927054328822122No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA22650.22916936476073302No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22630.22896700770575668No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19570.19800637829437287No Hit
GGCCAAAAGTGTACATAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTG19350.19578045068963285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC19270.1949710224697274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19210.19436395130479828No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19020.19244155928252282No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGATTCTGCTGATACCAATT18970.1919356666450819No Hit
ATCCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAG18780.19001327462280646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18610.18829323965550734No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.1874838114356019No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC18420.1863708476332319No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18390.18606731205076735No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17970.18181781389626367No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17810.18019895745645276No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17810.18019895745645276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17460.17665770899436636No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTATGTACACTTTTGGCCA17440.17645535193939002No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT17150.17352117464223274No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGA16960.17159878261995726No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16900.17099171145502817No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16850.17048581881758726No Hit
ATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA16600.1679563556303827No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16500.1669445703555009No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTATGTACA15740.15925500226639902No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGATTCTGCTGATACCAATTTAAAT15740.15925500226639902No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15700.15885028815644628No Hit
GGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTG15110.15288075503464352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15110.15288075503464352No Hit
TAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT15060.1523748623972026No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15060.1523748623972026No Hit
CAGTACCCCTATGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT14750.14923832804506898No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14730.14903597099009258No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14720.1489347924626044No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14230.14397704461568347No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13660.13820986854885708No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13610.13770397591141617No Hit
GTGTACATAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATC13520.13679336916402254No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13430.1358827624166289No Hit
GTACCCCTATGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGA13380.13537686977918797No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT12860.1301155863498025No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12830.12981205076733796No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12780.12930615812989704No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAAC12680.12829437285501522No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT12660.12809201580003884No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12660.12809201580003884No Hit
CTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGAT12640.1278896587450625No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12560.12708023052515702No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12550.12697905199766885No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12540.12687787347018067No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA12510.1265743378877161No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12490.12637198083273976No Hit
AGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGAATCCAGGGAAAGCCCCTAAGCTCCTGATCTATG12460.12606844525027522No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12410.1255625526128343No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12210.12353898206307065No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12170.12313426795311792No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12140.12283073237065337No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG12100.12242601826070064No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12040.12181894709577155No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12010.121515411513307No Hit
CAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA11910.12050362623842517No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG11650.11787298452373242No Hit
GTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAAT11620.11756944894126789No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT11560.1169623777763388No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11470.11605177102894516No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG11450.11584941397396878No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT11440.11574823544648061No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11420.11554587839150425No Hit
GTACATAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTT11400.11534352133652788No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11350.11483762869908697No Hit
GCTTGGTCCCCTGGCCAAAAGTGTACATAGGGGTACTGTAACTCTGTTGA11260.11392702195169332No Hit
GTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC11240.11372466489671697No Hit
CGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTG11010.11139755876448877No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11010.11139755876448877No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10930.1105881305445833No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTCTGCAAACT10890.11018341643463057No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10870.10998105937965422No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10530.10654098944505601No Hit
CCTATGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC10420.10542802564268601No Hit
AAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10350.10471977595026873No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10010.10127970601567053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2700.049.6101841
GTATCAA33950.043.298611
TCATGCG350.001374707839.26989422
TATCAAC38800.037.7958532
TGTGTCG4300.037.2232570
CAACGCA42300.034.8309175
GTACTAG4650.034.421570
TCAACGC43100.034.3437734
AACGCAG43100.034.1844066
GTCGCTA652.695953E-432.3895269
ACGCAGA46350.031.7874417
AGAGTAC44600.031.64703411
ATCAACG47050.031.46053
CGCAGAG47000.031.3478268
CAGAGTA48300.029.57823210
CCGCTAT1305.0526865E-528.41301370
TAGGACC854.586471E-628.2831084
TACGGGT4500.028.23678615
TTTTCGC500.007407189327.8455248
GGCGATA500.007685606427.63580338
Produced by FastQC (version 0.11.5)