FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310858
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61120.46625950331767435No Hit
GAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCAT49240.3756318380785714No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43720.33352201382605895No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41620.317501971990864No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC39830.30384679347419785No Hit
GTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTT37970.28965761356302516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37740.28790303755250374No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36930.28172387855892855No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTG36560.27890129975939426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35410.27012841970678747No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33500.25555781022811014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32670.24922607940753308No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32380.24701378791600614No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT29400.2242805856927295No Hit
GATCTATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA29280.22336515473071836No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28540.21771999713164966No Hit
GGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCA27890.2127614127540893No Hit
GTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA27450.20940483256004847No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26780.20429367635548626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26340.20093709616144542No Hit
GGTTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT26250.20025052293993706No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25370.19353736255185533No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC25260.19269821750334512No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAACTGAACGGGTTA25160.19193535836833586No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25160.19193535836833586No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAACTATTTAAATTGGTATCAGCAGAAA24970.1904859260118182No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGAATTAGCAA24920.19010449644431357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24860.189646780963308No Hit
GTTTAGTCCCCTGGCCAAAACTGAACGGGTTACTGTAACTCTGTTGACAG24820.1893416373093043No Hit
ACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGA23580.17988218403518919No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23550.1796533262946864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23510.17934818264068267No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23480.17911932490017987No Hit
ATGCTACATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA22010.1679052956155434No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21610.16485385907550626No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21460.16370957037299233No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA21300.1624889957569775No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20980.16004784652494777No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20680.15775926911991991No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAAC20100.15333468613686607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20060.15302954248286238No Hit
ACTTACTACTGTCAACAGAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA19770.15081725099133544No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19590.14944410454831872No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC19080.1455535229597714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18980.1447906638247621No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC18690.14257837233323517No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18490.1410526540632166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18460.14082379632271383No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA18330.13983207944720175No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17680.1348734950696414No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17530.13372920636712748No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA17100.13044891208658757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17010.1297623388650792No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16910.12899947973006992No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT16790.1280840487680588No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16590.12655833049804022No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA16510.12594804319003278No Hit
CCCCTAAGGTCCTGATCTATGCTACATCCAATTTACAAAGTGGGGTCCCA15850.12091317289897152No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15650.11938745462895294No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT15590.11892973914794738No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15380.11732773496442787No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG15320.11687001948342231No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA15310.11679373356992138No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAACCCGTTCAGTTTTGGCCAGGGGA15040.11473401390539632No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGTAAATT14770.11267429424087125No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14650.11175886327886012No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14630.11160629145185826No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14610.1114537196248564No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14370.10962285770083412No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14250.10870742673882297No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG13810.10535084654478212No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13750.10489313106377654No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13680.10435912966927005No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13640.10405398601526633No Hit
GTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA13580.10359627053426078No Hit
ACCTTGATGGGACCCCACTTTGTAAATTGGATGTAGCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG13560.10344369870725892No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13480.10283341139925148No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13420.10237569591824591No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13300.10146026495623478No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT13290.10138397904273384No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13260.10115512130223106No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13180.10054483399422363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32600.040.0186651
GTGGTAT4200.036.061551
TATCAAC37000.035.1626322
ATCAACG38500.033.792663
TCAACGC38550.033.7488334
CAACGCA38950.033.4906125
AACGCAG39300.033.1923456
ACGCAGA42300.030.756917
AGAGTAC42100.030.41250211
CGCAGAG43350.029.9325398
GCAGAGT44550.029.0490199
CAGAGTA46750.027.82924710
TGCCCGG14050.027.09877470
TTAGCTA658.6364406E-426.54965635
ACCGATC1457.185008E-1026.12549627
AATAGCG1951.8189894E-1225.24018962
GAGTACG38050.025.23718312
GAGTACT12700.025.20406212
AGTACGG38300.025.1632713
CAGTTCG27600.024.91972470
Produced by FastQC (version 0.11.5)