FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857163
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32300.3768244779581013No Hit
GTATATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGAATC30910.3606081923741459No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA30340.3539583486454735No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT21520.2510607667386483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21150.2467442015112645No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTATATTAGCAC20930.24417759515984708No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19250.2245780557490232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19230.2243447278988944No Hit
CTCCTGCAGGGCCAGTCAGTATATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT18670.21781154809528644No Hit
GTAATACACGGCCGTGTCTTCGGCAGTCACAGAGCTCAACTGCAGGGAGA18360.21419496641828917No Hit
GTGCAGCCACAGTTCGCCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17530.20451186063794166No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16920.1973953612090116No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16780.1957620662581096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16400.19132883710566137No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT16400.19132883710566137No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16210.1891122225294372No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAACAACTGGAATCTCACTTTCGGCGGAGGGA15750.18374568197647356No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14780.17242928124522408No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13920.1623961836896833No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13850.1615795362142323No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13840.16146287228916786No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA13560.15819628238736388No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13530.15784629061217062No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC13390.15621299566126862No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13370.15597966781113978No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13330.15551301211088206No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12340.14396328352950372No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTATATTAGCACCTACTTAGCCTGGTA12100.14116334932795746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12040.14046336577757088No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11970.13964671830211992No Hit
GGCCTGGAGTGGGTTGGGTACATCGATTACAGAGGGAACACCTACTACAA11850.13824675120134677No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11710.13661345625044477No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11690.13638012840031594No Hit
GTGTATTACTGTGCCAGAGGCCACTGGAACTACCCTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11550.13474683344941393No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATCTCCACCTTGGTCC11370.13264688279825423No Hit
TACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGAATCCAACAGGGCCA11310.13194689924786768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11230.13101358784735226No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC11220.13089692392228783No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10760.12553038336932415No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10410.12144714599206918No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10180.11876387571558736No Hit
CTCCCAGGCTCCTCATCTATGATGAATCCAACAGGGCCACTGGCATCCCA10050.11724724468974979No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10000.11666392506442765No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACACAGTC9780.11409731871301024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9430.11001408133575528No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9280.10826412245978885No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9250.10791413068459559No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA9140.10663082750888687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9110.1062808357336936No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8970.1046475407827916No Hit
GTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGATGGGCCTGGAGTGGGTTGGGTACATCGATTACAGAGGGAA8960.10453087685772718No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8950.10441421293266274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8950.10441421293266274No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8810.10278091798176076No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8790.1025475901316319No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8740.10196427050630977No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC8740.10196427050630977No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8670.10114762303085878No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC8660.10103095910579435No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8640.10079763125566549No Hit
GTTTATTACTGTCAGCAGCGTAACAACTGGAATCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGGCGAA8610.10044763948047221No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGAATCCAACAGGGCCACTGGCATCCCAGCCAGGTT8610.10044763948047221No Hit
CTAATATACTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA8590.10021431163034335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG200.00726268751.92163539
GTGGTAT1750.051.3131331
TGGTATC1800.049.8848572
GGTATCA6500.045.696021
TATATTC350.001320919139.58943642
GTATCAA14850.038.6077841
TTGTGAG7750.035.90624270
ATTCTAA400.00258284634.535676
TATCAAC17700.032.389392
TCAACGC18150.031.5863464
ATCAACG18250.031.413273
CAACGCA18450.031.0727485
AACGCAG18850.030.4133766
TGTTAGA3750.029.33735370
TGCGCTA900.006320325728.7621170
CGCAGAG20600.027.8280948
CCGACGC500.00756437427.72558243
TACACTC500.00769493527.6285385
AGAGTAC21300.026.75142511
GTGCTAT658.600286E-426.5674511
Produced by FastQC (version 0.11.5)