FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857163
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33540.3912908046660903No Hit
GTATATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGAATC27440.32012581037678944No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTACCA27200.3173258761752432No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT21730.2535107091650013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20320.237061095730917No Hit
GTGCAGCCACAGTTCGCCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20030.23367784190404858No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19760.23052791592730904No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTATATTAGCAC18480.2155949335190623No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17450.20357854923742624No Hit
GTAATACACGGCCGTGTCTTCGGCAGTCACAGAGCTCAACTGCAGGGAGA17280.20159526251133097No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16830.19634538588343176No Hit
CTCCTGCAGGGCCAGTCAGTATATTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT16370.19097884533046805No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT16100.18782891935372853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15940.18596229655269766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15890.18537897692737554No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15760.183862345901538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15550.181412403475185No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14680.17126264199457977No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAACAACTGGAATCTCACTTTCGGCGGAGGGA14250.1662460932168094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14140.1649627900411007No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14030.16367948686539202No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13080.15259641398427137No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12740.1486298405320808No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12620.1472298734313077No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12540.14629656203079228No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA12510.145946570255599No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12440.145129922780148No Hit
GGCCTGGAGTGGGTTGGGTACATCGATTACAGAGGGAACACCTACTACAA12090.14104668540289303No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11880.13859674297654004No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11730.13684678410057363No Hit
CTGCTACTCTGGCTCCCAGGTACCACCGGAGAAATTGTGTTGACACAGTC11710.13661345625044477No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTATATTAGCACCTACTTAGCCTGGTA11590.13521348914967166No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATCTCCACCTTGGTCC11540.13463016952434953No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11190.13054693214709454No Hit
GTGTATTACTGTGCCAGAGGCCACTGGAACTACCCTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11120.12973028467164355No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11020.12856364542099927No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11010.12844698149593484No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10940.12763033402048385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10410.12144714599206918No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10220.11923053141584507No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTAC10100.11783056431507193No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9900.11549728581378338No Hit
TACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGAATCCAACAGGGCCA9700.11316400731249483No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA9570.11164737628665726No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9470.110480737036013No Hit
CTCCCAGGCTCCTCATCTATGATGAATCCAACAGGGCCACTGGCATCCCA9210.10744747498433788No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8900.1038308933073406No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8850.10324757368201846No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8780.10243092620656749No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8750.1020809344313742No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8730.10184760658124535No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8710.10161427873111649No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8710.10161427873111649No Hit
GTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGATGGGCCTGGAGTGGGTTGGGTACATCGATTACAGAGGGAA8660.10103095910579435No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8580.10009764770527892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2700.049.889881
TGGTATC2950.045.6379282
GGTATCA7950.042.142111
GTATCAA17350.036.03221
TGTATAC400.002595655434.5008473
CACGGAT400.002600860534.4867557
TAGCATT400.002600860534.4867554
CTCCGCG800.003449086632.5533170
TATCAAC20200.030.9321782
ATCAACG20350.030.6862553
TCAACGC20250.030.484594
CAACGCA20800.029.6785075
AACGCAG21350.029.2370176
CGCAGAG22850.027.166818
TTGTGAG7450.027.09134570
AGAGTAC22550.027.07100911
GCAGAGT23700.026.0469599
ACGGGTG809.549868E-525.87865416
ACGCAGA24700.025.132057
CAGAGTA24300.025.11998410
Produced by FastQC (version 0.11.5)