FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960062
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.036.6211
GGTATCA14000.034.0466161
TATCAAC38500.030.4584142
ATCAACG38700.030.3896063
TCAACGC39500.029.7741224
CGCGTAG1052.4429028E-829.42801928
AACGCAG40300.029.0979926
CAACGCA40350.029.0619345
TCGTATG1202.5447662E-928.89387940
ACGCGTA1103.8526196E-828.08013227
ACGCAGA43100.027.20487
CGCAGAG44750.026.2030818
ACGGGTA2100.026.12281816
AGAGTAC45550.025.66760311
CGTTCAT700.001178361225.1718858
CAGAGTA47100.025.11409410
AGTACGG33500.024.76794213
GAGTACG33600.024.69422512
GCAGAGT47950.024.6689019
GTGGTAT5750.024.451371
Produced by FastQC (version 0.11.5)