FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603492
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23230.14487131834770614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA609.99592E-441.89797270
GTATCAA49500.040.099241
GGTATCA22350.035.4934271
TCAACGC57650.034.36064
CAACGCA57850.034.2407385
ATCAACG58050.034.138743
GTGGTAT8800.033.95081
AACGCAG58650.033.773696
TATCAAC59900.033.227882
ACGCAGA61800.032.052217
TGGTATC10000.031.2279032
CAGAGTA62700.031.15487110
AGAGTAC62550.031.0710311
CGCAGAG65250.030.4100198
GCAGAGT67500.029.345589
AGTACGG60250.027.71192213
GAGTACG60600.027.5492912
CGTAACT756.3755186E-527.4642526
TAATATC809.917436E-525.7372842
TATAGTC700.00138700924.4788275
Produced by FastQC (version 0.11.5)