FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608970
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.039.983171
TGGTATC3450.038.877922
GTGGTAT3850.037.549411
GGTATCA8150.037.165471
CGTTACC400.002576626434.5510336
ATCAACG24000.033.0887033
TATCAAC24150.032.8966982
TCAACGC24000.032.786064
CAACGCA24250.032.3063665
AACGCAG25000.031.4746176
ACGCAGA26750.029.4155317
CGCAGAG26850.029.3059758
AGAGTAC26750.028.39724211
CAGAGTA27400.027.7144810
GCAGAGT28950.026.9405619
GAGTACG22100.024.73340412
AGTACGG22400.024.40415813
TAATCGT1902.5465852E-1123.6655838
TTAATCG1902.5465852E-1123.64994237
CGTTGAA2103.6379788E-1223.15254442
Produced by FastQC (version 0.11.5)