FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942429
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC18930.20086393776082867No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16920.17953607115230963No Hit
GTTTAATACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15250.1618159033730923No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11350.1204334756252195No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10520.1116264461301594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10070.10685155062078948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9960.10568435394072127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9800.1039866133151675No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9520.10101556722044844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.038.6703571
GTATCAA29100.037.783471
TATCAAC32900.033.375052
TCAACGC33750.032.506874
ATCAACG33600.032.4524423
CAACGCA34600.031.7082925
AACGCAG35250.031.0260356
ACGCAGA37800.029.0239937
CAGAGTA39200.028.25062810
AGAGTAC38600.028.24576211
CGCAGAG39300.027.9162088
GCAGAGT40350.027.4440129
GGTATCA20500.027.0692461
CGGAGGG6650.023.30002828
AGTACGG32300.023.21444713
CGCGTAT1052.3342041E-522.93047712
GAGTACG32850.022.82577312
TTTCGGC6400.022.5781822
TTAATAC7400.022.311923
TAGACTG1554.2222382E-822.188435
Produced by FastQC (version 0.11.5)