FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329646
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11550.3503758577383011No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11430.34673558908647456No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10620.32216377568664567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8750.2654362558623493No Hit
GTGTAAGCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTT7900.23965101957857823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7840.237830885252665No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA7640.23176377083295413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7370.2235731663663445No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7340.2226630992033879No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7100.2153825618997349No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7090.21507920617874934No Hit
GTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGA6940.2105288703639662No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6850.20779866887509635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6730.20415840022326978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6610.2005181315714433No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCT6540.1983946415245445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6520.19778793008257342No Hit
CCCCAGAGACGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGGCCGA6230.1889906141739927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6220.18868725845300716No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA5990.18171007687033972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5700.17291276096175898No Hit
GGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCT5150.15622819630755416No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCCCAGGGCCGAAAGTG5130.15562148486558308No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5080.15410470626065537No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5050.15319463909769876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4980.15107114905079996No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4680.1419704774212337No Hit
GGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCA4500.13651007444349392No Hit
GAATTTACCCTCACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTT4440.13468994011758068No Hit
TCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCCTGGAAG4430.13438658439659515No Hit
CTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCA4370.13256645007068188No Hit
CACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTT4310.1307463157447686No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3990.12103893267323129No Hit
GCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTTTCTCCT3950.11982550978928912No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3820.11588188541647707No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3780.1146684625325349No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCTCTGGGTTCCAG3780.1146684625325349No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3760.11406175109056381No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3700.11224161676465057No Hit
ATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGC3660.1110281938807084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCC3640.11042148243873731No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3600.10920805955479515No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3550.10769128094986745No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3510.10647785806592526No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3490.10587114662395417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3480.10556779090296865No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3470.1052644351819831No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3440.10435436801902646No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC3430.10405101229804092No Hit
ATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCG3420.1037476565770554No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3350.1016241665301566No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3350.1016241665301566No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3320.10071409936719997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3320.10071409936719997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTA200.007471177351.54420526
GTCGTGA200.00748463851.5207516
GGTTATC200.00748463851.5207515
GTATCAA8250.048.73271
GTGGTAT507.145718E-848.1079181
TATCAAC9800.040.6681632
ACGGGAT2750.039.96760616
ATCAACG10700.037.247483
CAACGCA10750.037.0742345
TCAACGC10750.037.0742344
GGTATCA2700.036.9081951
AACGCAG10800.036.8969966
TTATACA601.3781413E-534.3575862
TACGGGA5250.034.02004615
ACGCAGA11750.033.9086887
CGCAGAG12150.032.7923558
GCAGAGT12250.032.5246629
CGGGATC751.7231614E-632.05735417
AGTACGG11250.031.75204313
GAGTACG11400.031.33425512
Produced by FastQC (version 0.11.5)