FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329646
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10690.3242872657335445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10480.3179167955928481No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8700.2639194772574216No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8190.24844833548715894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7630.23146041511196858No Hit
GTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGCCCTGGGA7370.2235731663663445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7350.22296645492437345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7190.21811276338860475No Hit
GTGTAAGCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTT7170.21750605194663367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7060.2141691390157927No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCA6950.21083222608495175No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA6370.1932375942677903No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCGTCT6230.1889906141739927No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6170.18717047984807947No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6060.1838335669172385No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6000.18201343259132524No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5990.18171007687033972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5940.180193298265412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5590.16957584803091802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5480.16623893510007706No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5440.16502551221613487No Hit
GAATTTACCCTCACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTT5400.1638120893321927No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCCCAGGGCCGAAAGTG5310.16108188784332284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5110.155014773423612No Hit
GGTTATAGCTTACACTGGTACCAGCAGAAACCAGATCGGCCTCCAAAGCT5050.15319463909769876No Hit
CTTTCGGCCCTGGGACGAAAGTGAATATCAAACGAACTGTGGCTGCACCA4930.14955437044587225No Hit
GGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCA4910.14894765900390117No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4550.13802685304842166No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4550.13802685304842166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.1362067187225084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4480.13590336300152286No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4370.13256645007068188No Hit
CACTATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTT4130.12528591276702886No Hit
CCCCAGAGACGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGGCCGA4090.12407248988308671No Hit
TCTCTGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCCTGGAAG4080.12376913416210115No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4010.12164564411520236No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACATCTCATTGGGTTTCTGCTGCTCTGGGTTCCAG3900.1183087311843614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3870.11739866402140477No Hit
ATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTACTGATTTTCCATACACTTTCGGC3820.11588188541647707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3790.11497181825352044No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC3780.1146684625325349No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3730.1131516839276072No Hit
GCTATAACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGATTTTCTCCT3690.11193826104366501No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTTACCCTCACTATCAATAGCC3590.10890470383380961No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3580.10860134811282406No Hit
ATTGATAGTGAGGGTAAATTCTGTCCCAGATCCACTGCCACTGAACCTCG3550.10769128094986745No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3530.10708456950789635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACTTTCGTCC3460.10496107946099756No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3430.10405101229804092No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3300.10010738792522889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTT309.004065E-657.6151939
GCGCAAA200.0074341251.6090431
TGGTATC1050.049.0693242
ACGGGTA451.916469E-645.7910816
GGTATCA3350.045.1419141
ACGAACC404.6317007E-543.678952
GTATCAA9300.043.238941
TAATCGT404.9488895E-543.19157838
GTGGTAT1200.042.961771
TCACGTA451.0207595E-438.19985225
TATCAAC10750.037.70352
CAACGCA11050.036.324885
ATCAACG11100.036.172233
TCAACGC11100.036.161254
AACGCAG11250.035.6791046
TCGGATT400.002281400435.4138562
TTAATCG501.8434008E-434.54798537
ATCTTAC400.002642334234.3694151
ATCGTTG751.6268914E-632.29413240
TAGCGGC552.7411146E-432.2905164
Produced by FastQC (version 0.11.5)