FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033158
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44940.4349770315866499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35000.3387671585565809No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30610.2962760778119126No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29970.2900814783411637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29190.28253181023618845No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC29110.28175748530234485No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA28900.27972488235100534No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28650.27730511693274407No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27440.2655934523083594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26850.2598828059212627No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26140.2530106721334007No Hit
GAGTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24100.23326538632038857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23770.23007129596828366No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT23020.22281199971349977No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22740.22010186244504712No Hit
GTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA22020.21313293804045458No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21960.21255219434007191No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21310.20626080425259255No Hit
GTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA20720.20055015786549588No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20610.19948546108146092No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAG20100.19454913962820788No Hit
GTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT20070.19425876777801654No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20010.19367802407763382No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19970.19329086161071202No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19960.19319407099398156No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19780.19145183989283343No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18300.17712682861672657No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT18020.17441669134827392No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17970.17393273826462166No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17510.1694803698950209No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17180.166286279542916No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17160.16609269830945508No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17080.16531837337561148No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTTCGGCCAAGGGA16970.1642536765915765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16910.1636729328911938No Hit
TTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16830.1628986079573502No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA16460.15931735513832346No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15360.14867038729797377No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT15300.14808964359759108No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACC15050.14566987817932978No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14890.14412122831164256No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC14880.1440244376949121No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14640.14170146289338126No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTT14230.13773304760743274No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGA14220.13763625699070228No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14040.13589402588955415No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13790.13347426047129288No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA13650.13211919183706655No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13440.13008658888572705No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13430.1299897982689966No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13320.12892510148496164No Hit
GTCCAGGGGGTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTC13240.12815077655111803No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13090.12669891730016125No Hit
GAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG13080.12660212668343082No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12760.12350482694805634No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12730.123214455097865No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG12640.12234333954729093No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT12580.12176259584690823No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12520.1211818521465255No Hit
ACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12240.11847171487807287No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT11980.11595515884308112No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCT11850.11469688082558524No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11780.11401934650847208No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11630.11256748725751531No Hit
ACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11630.11256748725751531No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11620.11247069664078485No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11490.111212418623289No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11480.11111562800655854No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTG11400.11034130307271492No Hit
GGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11330.10966376875560174No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11210.10850228135483633No Hit
GGGTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT11190.10830870012137543No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11080.10724400333734045No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCA11070.10714721272061002No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA10830.10482423791907916No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10820.10472744730234873No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10750.10404991298523555No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10670.10327558805139193No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTA10560.10221089126735698No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10550.10211410065062652No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10470.10133977571678292No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10450.101146194483322No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10440.10104940386659156No Hit
CCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAG10390.1005654507829393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC200.007363462451.7417435
GTATCAA20400.041.3511
TCAACGC23950.035.2166144
TATCAAC24100.034.997422
GTGGTAT3350.034.9449351
ATCAACG24500.034.4260373
CAACGCA24700.034.147285
AACGCAG25400.033.204616
ACGCAGA27500.030.6660187
AGAGTAC27500.030.66453211
CGCAGAG27700.030.44468
GCAGAGT28600.029.6054769
GAGTGAT3400.028.96208870
CATAACG500.00781598627.540832
TGTTAGA9050.026.52191270
CGAGTGA2350.026.41926669
CGTATGC1057.502913E-726.35694341
CAGAGTA32900.025.73606710
TGTCGAG2250.025.36601366
TCGTATG1101.1271532E-625.15522840
Produced by FastQC (version 0.11.5)