FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254844
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58850.037.0447461
GTGGTAT12550.036.5564461
GGTATCA26750.034.1726041
TATCAAC66350.032.468152
ATCAACG68400.031.6188553
TCAACGC68600.031.5203824
CAACGCA69750.031.051435
AACGCAG70850.030.8616186
TGGTATC15750.029.3253152
ACGCAGA77000.028.3519787
AGAGTAC79850.027.16995811
CGCAGAG80750.027.035328
CAGAGTA82950.026.27736510
GCAGAGT86150.025.4200869
CGATTAA2001.2442488E-624.8723170
AGTACGG68500.023.1285513
GAGTACG70300.022.68182212
AGTACTT29850.019.49950213
GAGTACT30050.019.36843312
GTACGGG82850.019.0398614
Produced by FastQC (version 0.11.5)