FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501074
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCTCCACCC80270.5347504520096944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67360.4487453649853371No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA62250.4147030726000184No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT52410.3491500085938468No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT44200.2944558362878845No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43440.28939279475895263No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43350.28879322405157903No Hit
GATCAGGACTCCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC41570.2769350478390806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40490.2697401993505983No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39800.265143490594068No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA39450.26281182673205983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38960.25954749732524846No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGAATCAGGGAGAACTGGTTCTTAGACGTGTCTTCTGA37650.2508204125845894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37620.2506205556821316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37590.2504206987796737No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGCTTACTTCTGGAGCTGGATCCG34060.22690420325713456No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33830.2253719670049578No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31400.20918355790587273No Hit
GGACTCCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31140.20745146475123813No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30910.20591922849906133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30060.2002566162627559No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29930.19939056968543856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29650.19752523859583207No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26360.17560759829295558No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGT26330.17540774139049772No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA26070.17367564823586312No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA25950.17287622062603175No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC25660.1709442705689393No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACTCTCC25570.17034469986156578No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24700.1645488496902884No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24650.1642157548528587No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24070.16035185473867378No Hit
CCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23790.15848652364906726No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA23480.15642133565700292No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23450.1562214787545451No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA23210.15462262353488235No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACT22800.15189124586795855No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGA22770.1516913889655007No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22430.14942634407097852No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22170.14769425091634392No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC21850.1455624439567936No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG21540.14349725596472926No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGCTTACTTCTGGA21490.14316416112729952No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21320.14203163868003843No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21180.14109897313523517No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20770.13836759546831137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20640.13750154889099406No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20500.1365688833461908No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC20250.13490340915904211No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20220.13470355225658429No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19940.13283822116697777No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19830.13210541252463237No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19790.13183893665468857No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA19510.12997360556508206No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19220.1280416555079896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19170.12770856067055988No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19040.12684251409324257No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT18920.12604308648341123No Hit
CCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18800.12524365887357983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18740.12484394506866417No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAAGATCT18740.12484394506866417No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18650.12424437436129064No Hit
GTATATTACTGTGCGAGAGAGACGGTGACTACGGGCGGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT18200.12124652082442305No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC18090.12051371218207764No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17520.11671643103537868No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17420.11605024136051921No Hit
GCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGG17350.11558390858811757No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA17100.11391843440096891No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17010.1133188636935954No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16880.11245281711627808No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16830.11211972227884834No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16740.11152015157147481No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC16670.11105381879907321No Hit
GTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT16620.11072072396164345No Hit
GTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGC16560.1103210101567278No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16510.10998791531929804No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16430.10945496357941047No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16330.10878877390455102No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCCTCTCACA16240.1081892031971775No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16220.10805596526220561No Hit
CAGTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG15990.10652372901002881No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15550.10359249444064715No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15540.10352587547316121No Hit
TTATTACTGCATGCAAGGTACACACTGGCCGTACACTTTTGGCCAGGGGA15450.1029263047657877No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTC15140.10086111677372336No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15140.10086111677372336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)