FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501074
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCTCCACCC94490.6294826237747106No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63450.42269734869833203No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA49470.32956403215297847No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47350.3154408110459578No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT42640.28406327736007686No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42170.2809321858882374No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA39570.2636112543418912No Hit
GATCAGGACTCCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC39480.26301168363451766No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT39380.2623454939596582No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGAATCAGGGAGAACTGGTTCTTAGACGTGTCTTCTGA38370.2556169782435776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37890.25241926780425217No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37680.25102026948704725No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37170.24762270214526397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35070.2336327189732152No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGCTTACTTCTGGAGCTGGATCCG34170.22763701189948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33390.22244073243557613No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG33050.22017568754105393No Hit
GGACTCCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31620.21064917519056356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30000.19985690245784019No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29460.19625947821359907No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29300.19519357473382393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29140.19412767125404876No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA28170.18766563140791193No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27930.1860667761882492No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27620.18400158819618487No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26370.17567421726044152No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA25580.17041131882905172No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC25510.16994498605665012No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA25340.168812463609389No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24040.16015199783621592No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23570.15702090636437643No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC22970.15302376831521963No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG22620.1506921044532115No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGT22600.1505588665182396No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22190.1478274888513158No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22080.14709468020897037No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACTCTCC21980.14642849053411092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21880.14576230085925143No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21570.1436971128671871No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21050.14023292655791786No Hit
CCTCAGTTCACCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20770.13836759546831137No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA20450.13623578850876106No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC20190.13450369535412643No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGCTTACTTCTGGA20160.1343038384516686No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20050.1335710298093232No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19790.13183893665468857No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19540.1301734624675399No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19440.12950727279268043No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACT19400.12924079692273666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19330.12877446415033503No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19110.1273088468656442No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGA19040.12684251409324257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18760.12497718300363606No Hit
GTATATTACTGTGCGAGAGAGACGGTGACTACGGGCGGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT18520.12337832778397334No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18480.12311185191402957No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC17800.11858176212498517No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17780.11844852419001328No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT17720.1180488103850976No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC17650.11758247761269598No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17560.11698290690532245No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17390.11585038445806135No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAAGATCT17320.11538405168565974No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16930.11278591195370781No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16810.11198648434387645No Hit
GGGTGGAGGCTGAGGATATTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCGTACACTTTTGGCCAGGGGA16650.11092058086410131No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16610.11065410499415751No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16570.11038762912421374No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC16510.10998791531929804No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16430.10945496357941047No Hit
CAGTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG16400.10925510667695262No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16320.10872215493706505No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA16190.10785610835974775No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16090.10718991868488828No Hit
GCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGG16070.1070566807499164No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA15560.10365911340813311No Hit
TTATTACTGCATGCAAGGTACACACTGGCCGTACACTTTTGGCCAGGGGA15550.10359249444064715No Hit
CCCTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT15310.10199363922098444No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCCTCTCACA15220.10139406851361092No Hit
GTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGC15190.10119421161115308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3300.046.0040051
TGGTATC3500.044.331422
GTATCAA26300.043.423931
GGTATCA10350.043.0037421
TATCAAC30900.036.823292
ATCAGTG34950.034.5700970
ATCAACG33250.034.0943073
CAACGCA33400.033.927625
TCAACGC33450.033.8769074
AACGCAG34400.033.241736
CGCAGAG37200.030.5544838
AGAGTAC37550.030.36546111
CAGAGTA38100.030.01453210
TTAACGG1052.3081157E-829.6026535
GCAGAGT39250.029.2219059
ACGCAGA40050.028.4661967
CGTGTTG900.00688802428.26256870
TTTAACG1103.6379788E-828.24858334
GTGTATG1503.45608E-1127.6024021
CGAATGT500.007798066427.5545884
Produced by FastQC (version 0.11.5)