FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335234
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.040.4160841
GGTATCA12050.038.9799461
GTGGTAT3850.036.7800671
ATCAACG32850.034.5834733
TCAACGC33300.033.9087374
CAACGCA33600.033.605985
AACGCAG34500.032.829396
TATCAAC34950.032.3091052
CGCTTAT450.00460190230.69529524
ACGCAGA37450.030.2422317
CAGAGTA38200.029.73886110
AGAGTAC38250.029.69998711
CGCAGAG38250.029.519448
TGGTATC4950.028.6024322
GCAGAGT40400.027.948489
ACGCGTG500.007637093327.672435
AATAGCG3250.024.7740462
AACCGAT851.4321739E-424.38115528
AGTACGG37900.023.68786613
GAGTACG38400.023.4693512
Produced by FastQC (version 0.11.5)